Publications
Jungbauer A., Satzer P., Duerauer A., Azevedo A., Aires-Barros R., Nilsson B., Farid S., Goldrick S., Ottens M., Sponchioni M., Fernandez Lahore H.M.
Continuous downstream processing, Separation and Purification Technology 338 (2024) 126439
https://doi.org/10.1016/j.seppur.2024.126439
Wahab M.A., Domingues C., Azevedo A.M., Chu V., Conde J.P., Aires-Barros R. An integrated microfluidic device for continuous bioprocessing, Separation and Purification Technology 332, 2024
https://doi.org/10.1016/j.seppur.2023.125702
Isaksson M., Gomis-Fons J., Andersson N., Nilsson B. An automated buffer management system for small-scale continuous downstream bioprocessing, Journal of Chromatography A 1695, 2023 https://doi.org/10.1016/j.chroma.2023.463942
Montes-Serrano I., Komuczki D.P., Dürauer A. A modelling approach for volumetric power input in the microscale and its utilization for the scale-up of solid-liquid mixing systems, Chemical Engineering and Processing: Process Intensification 184, 2023 https://doi.org/10.1016/j.cep.2023.109303
Andersson N., Gomis Fons J., Isaksson M., Tallvod S., Espinoza D., Sjökvist L., Zandler Andersson G., Nilsson B. Methodology for fast development of digital solutions in integrated continuous downstream processing, Biotechnology and Bioengineering, 2023 https://doi.org/10.1002/bit.28501
São Pedro, M.N., Isaksson, M., Gomis-Fons, J., Eppink M.H.M., Nilsson, B. & Ottens Real-time detection of mAb aggregates in an integrated downstream process, Biotechnology & Bioengineering 120 (10), 2023 https://doi.org/10.1002/bit.28466
São Pedro, M.N., Eppink M.H.M., & Ottens, M Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregates, Biotechnology & Bioengineering, e3355, 2023 https://doi.org/10.1002/btpr.3355
Medeiros Garcia Alcântara J.; Iannacci F.; Morbidelli M., Sponchioni M. Soft Sensor based on Raman Spectroscopy for the In-Line Monitoring of Metabolites and Polymer Quality in the Biomanufacturing of Polyhydroxyalkanoates, Journal of Biotechnology 377 (20), 2023 https://doi.org/10.1016/j.jbiotec.2023.10.005
Guo Y., Kangwa M., Ali W., Mayer-Gall T., Gutmann J.S., Zenneck C., Winter M., Jungbauer A., Fernandez Lahore H.M. Moving adsorption belt system for continuous bioproduct recovery utilizing composite fibrous adsorbents, Frontiers in Bioengineering and Biotechnology 11, 2023 https://doi.org/10.3389/fbioe.2023.1135447
Herlevi L.-M., Fernandez-Lahore M., Ferreira G. A fluidized-bed-riser adsorption system for continuous bioproduct recovery from crude feedstock, Biotechnology and Bioengineering 120 (10), 2023 https://doi.org/10.3389/fbioe.2023.1135447
Herlevi L.-M., Fernandez Lahore H.M. Potential of fluidized bed adsorption technology in continuous bioproduct recovery and purification, Advances in Chemical Engineering, 59 (1), 2022 https://doi.org/10.1016/bs.ache.2022.03.006
Pons Royo, M.D.C., De Santis, T., Komuczki, D., Satzer, P., Jungbauer, A. Precipitation of antibodies by feeding of solid polyethylene glycol, Separation and Purification Technology 304, 122373, 2023 10.1016/j.seppur.2022.122373
Ronin, L,. Dotelli, G. Life Cycle Assessment Overview on Polyhydroxyalkanoates, Innovazione e circolarità, Il contributo del Life Cycle Thinking nel Green Deal per la neutralità climatica. Italia: Associazione Rete Italiana LCA, pp. 358–365, 2022.
São Pedro, M.N., Santos, M.S., Eppink, M.H.M., Ottens, M. Design of a microfluidic mixer channel: First steps into creating a fluorescent dye-based biosensor for mAb aggregate detection, Biotechnology Journal, 2022 https://doi.org/10.1002/biot.202200332
Eslami, T., Steinberger, M., Csizmazia, C., Jungbauer, A., Lingg, N. Online optimization of dynamic binding capacity and productivity by model predictive control, Journal of Chromatography A, 1680, 2022 https://doi.org/10.1016/j.chroma.2022.463420
Eslami T., Jakob, L.A., Satzer P., Ebner G., Jungbauer A., Lingg N. Productivity for free: Residence time gradients during loading increase dynamic binding capacity and productivity, Separation and Purification Technology, 281, 2022 https://doi.org/10.1016/j.seppur.2021.119985
Guo, Y., Ali, W., Schneider, A., Salma, A., Mayer-Gall, T., Gutmann, J.S., Fernandez Lahore, H.M. Megaporous monolithic adsorbents for bioproduct recovery as prepared on the basis of nonwoven fabrics, Electrophoresis, 43 (13-14), 2022 https://doi.org/10.1002/elps.202100220
Pons Royo, M.D.C., Montes-Serrano, I., Valery, E., Jungbauer, A., Satzer, P. Milliscale reactors for integration of continuous precipitation and filtration, Journal of Chemical Technology and Biotechnology, 2022 https://doi.org/10.1002/jctb.7187
Pons Royo, M.d.C., Beulay, J.-L., Valery, E., Jungbauer, A., Satzer, P. Design of millidevices to expedite apparent solubility measurements Reaction, Chemistry and Engineering, 2022 https://doi.org/10.1039/d2re00022a
Pons Royo, M.d.C., Beulay, J.-L., Valery, E., Jungbauer, A., Satzer, P. Mode and dosage time in polyethylene glycol precipitation process influences protein precipitate size and filterability, Process Biochemistry, Volume 114, 2022 https://doi.org/10.1016/j.procbio.2022.01.017
Lali N., Satzer P., Jungbauer A. Residence time distribution in counter-current protein A affinity chromatography using an inert tracer, Journal of Chromatography A, Volume 1683, 2022 https://doi.org/10.1016/j.chroma.2022.463530
Silva, T.C., Eppink, M., Ottens, M. Small, smaller, smallest: Miniaturization of chromatographic process development, Journal of Chromatography A, 1681, 2022 https://doi.org/10.1016/j.chroma.2022.463451
J. Scheffel, M. Isaksson, J. Gomis-Fons, H. Schwarz, N. Andersson, B. Norén, A. Solbrand, V. Chotteau, S. Hober, B. Nilsson Design of an integrated continuous downstream process for acid-sensitive monoclonal antibodies based on a calcium-dependent Protein A ligand, Journal of Chromatography A, 1664, 2022 https://doi.org/10.1016/j.chroma.2022.462806
I. Montes-Serrano, P. Satzer, A. Jungbauer, A. Dürauer Characterization of hydrodynamics and volumetric power input in microtiter plates for the scale-up of downstream operations (2022), Biotechnology and Bioengineering, 119 (2), 2022 https://doi.org/10.1002/bit.27983
A. Javidanbardan, V. Chu, J.P. Conde, A.M. Azevedo Microchromatography integrated with impedance sensor for bioprocess optimization: Experimental and numerical study of column efficiency for evaluation of scalability Journal of Chromatography A, 1661, 2022 https://doi.org/10.1016/j.chroma.2021.462678
H. Schwarz, J. Gomis-Fons, M. Isaksson, J. Scheffel, N. Andersson, A. Andersson, A. Castan, A. Solbrand, S. Hober, B. Nilsson, V. Chotteau Integrated continuous biomanufacturing on pilot scale for acid-sensitive monoclonal antibodies Biotechnology and Bioengineering, 2022 https://doi.org/10.1002/bit.28120
A. Javidanbardan, A.M. Azevedo, V. Chu, J.P. Conde A systematic approach for developing 3d high-quality pdms microfluidic chips based on micromilling technology, Micromachines, 13 (1), 2022 https://doi.org/10.3390/mi13010006
Lali N., Jungbauer A., Satzer P. Traceability of products and guide for batch definition in integrated continuous biomanufacturing, Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6953
Armstrong, K. Horry, T. Cui, M. Hulley, R. Turner, S. S. Farid, S. Goldrick, D. G. Bracewell Advanced control strategies for bioprocess chromatography: Challenges and opportunities for intensified processes and next generation products, Journal of Chromatography A, Volume 1639, 2021, 461914, https://doi.org/10.1016/j.chroma.2021.461914
N. São Pedro, T.C. Silva, P. Rohan, M. Ottens White paper on high-throughput process development for integrated continuous biomanufacturing Biotechnology and Bioengineering, 2021 https://doi.org/10.1002/bit.27757
T.C. Silva, M. Eppink, M. Ottens Automation and miniaturization: enabling tools for fast, high-throughput process development in integrated continuous biomanufacturing, Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6792
Yelemane, M. Kangwa, R.N. Dsouza, M. Fernández-Lahore Surface energetics to assess influence of biomass-type and biomass–adsorbent interactions in expanded beds, Bioresources and Bioprocessing, 8 (1), art. no. 29, 2021 https://doi.org/10.1186/s40643-021-00382-6
M.N. São Pedro, M.E. Klijn, M.H.M. Eppink, M. Ottens Process analytical technique (PAT) miniaturization for monoclonal antibody aggregate detection in continuous downstream processing Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6920
Schneider A., Herlevi L.-M., Guo,Y., Fernández Lahore, H.M. Perspectives on adsorption technology as an effective strategy for continuous downstream bioprocessing, Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6923
Medeiros Garcia Alcântara, F. Distante, G. Storti, D. Moscatelli, M. Morbidelli, M. Sponchioni Current trends in the production of biodegradable bioplastics: The case of polyhydroxyalkanoates, Biotechnology Advances, Volume 42, 2020, 107582, https://doi.org/10.1016/j.biotechadv.2020.107582
https://doi.org/10.1016/j.seppur.2024.126439
Wahab M.A., Domingues C., Azevedo A.M., Chu V., Conde J.P., Aires-Barros R. An integrated microfluidic device for continuous bioprocessing, Separation and Purification Technology 332, 2024
https://doi.org/10.1016/j.seppur.2023.125702
Isaksson M., Gomis-Fons J., Andersson N., Nilsson B. An automated buffer management system for small-scale continuous downstream bioprocessing, Journal of Chromatography A 1695, 2023 https://doi.org/10.1016/j.chroma.2023.463942
Montes-Serrano I., Komuczki D.P., Dürauer A. A modelling approach for volumetric power input in the microscale and its utilization for the scale-up of solid-liquid mixing systems, Chemical Engineering and Processing: Process Intensification 184, 2023 https://doi.org/10.1016/j.cep.2023.109303
Andersson N., Gomis Fons J., Isaksson M., Tallvod S., Espinoza D., Sjökvist L., Zandler Andersson G., Nilsson B. Methodology for fast development of digital solutions in integrated continuous downstream processing, Biotechnology and Bioengineering, 2023 https://doi.org/10.1002/bit.28501
São Pedro, M.N., Isaksson, M., Gomis-Fons, J., Eppink M.H.M., Nilsson, B. & Ottens Real-time detection of mAb aggregates in an integrated downstream process, Biotechnology & Bioengineering 120 (10), 2023 https://doi.org/10.1002/bit.28466
São Pedro, M.N., Eppink M.H.M., & Ottens, M Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregates, Biotechnology & Bioengineering, e3355, 2023 https://doi.org/10.1002/btpr.3355
Medeiros Garcia Alcântara J.; Iannacci F.; Morbidelli M., Sponchioni M. Soft Sensor based on Raman Spectroscopy for the In-Line Monitoring of Metabolites and Polymer Quality in the Biomanufacturing of Polyhydroxyalkanoates, Journal of Biotechnology 377 (20), 2023 https://doi.org/10.1016/j.jbiotec.2023.10.005
Guo Y., Kangwa M., Ali W., Mayer-Gall T., Gutmann J.S., Zenneck C., Winter M., Jungbauer A., Fernandez Lahore H.M. Moving adsorption belt system for continuous bioproduct recovery utilizing composite fibrous adsorbents, Frontiers in Bioengineering and Biotechnology 11, 2023 https://doi.org/10.3389/fbioe.2023.1135447
Herlevi L.-M., Fernandez-Lahore M., Ferreira G. A fluidized-bed-riser adsorption system for continuous bioproduct recovery from crude feedstock, Biotechnology and Bioengineering 120 (10), 2023 https://doi.org/10.3389/fbioe.2023.1135447
Herlevi L.-M., Fernandez Lahore H.M. Potential of fluidized bed adsorption technology in continuous bioproduct recovery and purification, Advances in Chemical Engineering, 59 (1), 2022 https://doi.org/10.1016/bs.ache.2022.03.006
Pons Royo, M.D.C., De Santis, T., Komuczki, D., Satzer, P., Jungbauer, A. Precipitation of antibodies by feeding of solid polyethylene glycol, Separation and Purification Technology 304, 122373, 2023 10.1016/j.seppur.2022.122373
Ronin, L,. Dotelli, G. Life Cycle Assessment Overview on Polyhydroxyalkanoates, Innovazione e circolarità, Il contributo del Life Cycle Thinking nel Green Deal per la neutralità climatica. Italia: Associazione Rete Italiana LCA, pp. 358–365, 2022.
São Pedro, M.N., Santos, M.S., Eppink, M.H.M., Ottens, M. Design of a microfluidic mixer channel: First steps into creating a fluorescent dye-based biosensor for mAb aggregate detection, Biotechnology Journal, 2022 https://doi.org/10.1002/biot.202200332
Eslami, T., Steinberger, M., Csizmazia, C., Jungbauer, A., Lingg, N. Online optimization of dynamic binding capacity and productivity by model predictive control, Journal of Chromatography A, 1680, 2022 https://doi.org/10.1016/j.chroma.2022.463420
Eslami T., Jakob, L.A., Satzer P., Ebner G., Jungbauer A., Lingg N. Productivity for free: Residence time gradients during loading increase dynamic binding capacity and productivity, Separation and Purification Technology, 281, 2022 https://doi.org/10.1016/j.seppur.2021.119985
Guo, Y., Ali, W., Schneider, A., Salma, A., Mayer-Gall, T., Gutmann, J.S., Fernandez Lahore, H.M. Megaporous monolithic adsorbents for bioproduct recovery as prepared on the basis of nonwoven fabrics, Electrophoresis, 43 (13-14), 2022 https://doi.org/10.1002/elps.202100220
Pons Royo, M.D.C., Montes-Serrano, I., Valery, E., Jungbauer, A., Satzer, P. Milliscale reactors for integration of continuous precipitation and filtration, Journal of Chemical Technology and Biotechnology, 2022 https://doi.org/10.1002/jctb.7187
Pons Royo, M.d.C., Beulay, J.-L., Valery, E., Jungbauer, A., Satzer, P. Design of millidevices to expedite apparent solubility measurements Reaction, Chemistry and Engineering, 2022 https://doi.org/10.1039/d2re00022a
Pons Royo, M.d.C., Beulay, J.-L., Valery, E., Jungbauer, A., Satzer, P. Mode and dosage time in polyethylene glycol precipitation process influences protein precipitate size and filterability, Process Biochemistry, Volume 114, 2022 https://doi.org/10.1016/j.procbio.2022.01.017
Lali N., Satzer P., Jungbauer A. Residence time distribution in counter-current protein A affinity chromatography using an inert tracer, Journal of Chromatography A, Volume 1683, 2022 https://doi.org/10.1016/j.chroma.2022.463530
Silva, T.C., Eppink, M., Ottens, M. Small, smaller, smallest: Miniaturization of chromatographic process development, Journal of Chromatography A, 1681, 2022 https://doi.org/10.1016/j.chroma.2022.463451
J. Scheffel, M. Isaksson, J. Gomis-Fons, H. Schwarz, N. Andersson, B. Norén, A. Solbrand, V. Chotteau, S. Hober, B. Nilsson Design of an integrated continuous downstream process for acid-sensitive monoclonal antibodies based on a calcium-dependent Protein A ligand, Journal of Chromatography A, 1664, 2022 https://doi.org/10.1016/j.chroma.2022.462806
I. Montes-Serrano, P. Satzer, A. Jungbauer, A. Dürauer Characterization of hydrodynamics and volumetric power input in microtiter plates for the scale-up of downstream operations (2022), Biotechnology and Bioengineering, 119 (2), 2022 https://doi.org/10.1002/bit.27983
A. Javidanbardan, V. Chu, J.P. Conde, A.M. Azevedo Microchromatography integrated with impedance sensor for bioprocess optimization: Experimental and numerical study of column efficiency for evaluation of scalability Journal of Chromatography A, 1661, 2022 https://doi.org/10.1016/j.chroma.2021.462678
H. Schwarz, J. Gomis-Fons, M. Isaksson, J. Scheffel, N. Andersson, A. Andersson, A. Castan, A. Solbrand, S. Hober, B. Nilsson, V. Chotteau Integrated continuous biomanufacturing on pilot scale for acid-sensitive monoclonal antibodies Biotechnology and Bioengineering, 2022 https://doi.org/10.1002/bit.28120
A. Javidanbardan, A.M. Azevedo, V. Chu, J.P. Conde A systematic approach for developing 3d high-quality pdms microfluidic chips based on micromilling technology, Micromachines, 13 (1), 2022 https://doi.org/10.3390/mi13010006
Lali N., Jungbauer A., Satzer P. Traceability of products and guide for batch definition in integrated continuous biomanufacturing, Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6953
Armstrong, K. Horry, T. Cui, M. Hulley, R. Turner, S. S. Farid, S. Goldrick, D. G. Bracewell Advanced control strategies for bioprocess chromatography: Challenges and opportunities for intensified processes and next generation products, Journal of Chromatography A, Volume 1639, 2021, 461914, https://doi.org/10.1016/j.chroma.2021.461914
N. São Pedro, T.C. Silva, P. Rohan, M. Ottens White paper on high-throughput process development for integrated continuous biomanufacturing Biotechnology and Bioengineering, 2021 https://doi.org/10.1002/bit.27757
T.C. Silva, M. Eppink, M. Ottens Automation and miniaturization: enabling tools for fast, high-throughput process development in integrated continuous biomanufacturing, Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6792
Yelemane, M. Kangwa, R.N. Dsouza, M. Fernández-Lahore Surface energetics to assess influence of biomass-type and biomass–adsorbent interactions in expanded beds, Bioresources and Bioprocessing, 8 (1), art. no. 29, 2021 https://doi.org/10.1186/s40643-021-00382-6
M.N. São Pedro, M.E. Klijn, M.H.M. Eppink, M. Ottens Process analytical technique (PAT) miniaturization for monoclonal antibody aggregate detection in continuous downstream processing Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6920
Schneider A., Herlevi L.-M., Guo,Y., Fernández Lahore, H.M. Perspectives on adsorption technology as an effective strategy for continuous downstream bioprocessing, Journal of Chemical Technology and Biotechnology, 2021 https://doi.org/10.1002/jctb.6923
Medeiros Garcia Alcântara, F. Distante, G. Storti, D. Moscatelli, M. Morbidelli, M. Sponchioni Current trends in the production of biodegradable bioplastics: The case of polyhydroxyalkanoates, Biotechnology Advances, Volume 42, 2020, 107582, https://doi.org/10.1016/j.biotechadv.2020.107582
Movies
Video demonstration
Microfluidic Chip device for fast at-line PAT for high-molecular-weight species. Provided by Mariana Neves São Pedro from TU Delft.
Video demonstration
Integrated continuous downstream processing of monoclonal antibodies. Provided by Madelène Isaksson from Lund University.
CODOBIO Expert Interviews
The ICB V conference was also used to talk to experts and pioneers from global biopharmaceutical companies active in the field of continuous downstream processing about their view on the contribution of well-trained young scientists to the advancement of the field. You can watch here the interviews that CODOBIO coordinator Alois Jungbauer held with three leading experts: Kevin Brower (Sanofi), Chetan Goudar (Amgen) and Konstantin Konstantinov (Codiak Biosciences). A longer version also contains their views on continuous bioprocessing in general.
European Researchers Night 2020
Ignacio Monte-Serrano works in the MSCA-ITN project Codobio! He tells us about his work as a PhD student.
Blog Articles
Some CODOBIO fellows have presented their work in blog articles on the science blog hosted by acib
L. Ronin, Politecnico di Milano Continuous downstream production of polyhydroxyalkanoates, a family of biodegradable plastics
M. Isaksson, Lund University “Smart-factories” for continuous downstream processing of antibodies
L. Ronin, Politecnico di Milano Continuous downstream production of polyhydroxyalkanoates, a family of biodegradable plastics
M. Isaksson, Lund University “Smart-factories” for continuous downstream processing of antibodies
